TSLP Stable Expression Cell Line (A549)

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  • 细胞图0 TSLP Stable Expression Cell Line (A549)-细胞图0
  • 细胞图1 TSLP Stable Expression Cell Line (A549)-细胞图1
  • 质粒图0 TSLP Stable Expression Cell Line (A549)-质粒图0
  • 质粒图1 TSLP Stable Expression Cell Line (A549)-质粒图1
  • TSLP Stable Expression Cell Line (A549) TSLP Stable Expression Cell Line (A549)-产品图
细胞图0 TSLP Stable Expression Cell Line (A549)-细胞图0
细胞图1 TSLP Stable Expression Cell Line (A549)-细胞图1
质粒图0 TSLP Stable Expression Cell Line (A549)-质粒图0
质粒图1 TSLP Stable Expression Cell Line (A549)-质粒图1
TSLP Stable Expression Cell Line (A549) TSLP Stable Expression Cell Line (A549)-产品图
TSLP Stable Expression Cell Line (A549) Copy Product Info
Catalog#： YOE-L064
Size： 1*10^6
Culture method： 90% F12K+10% FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 TSLP Stable Expression Cell Line (A549)-细胞图0
  • 细胞图1 TSLP Stable Expression Cell Line (A549)-细胞图1
  • 质粒图0 TSLP Stable Expression Cell Line (A549)-质粒图0
  • 质粒图1 TSLP Stable Expression Cell Line (A549)-质粒图1
  • TSLP Stable Expression Cell Line (A549) TSLP Stable Expression Cell Line (A549)-产品图
TSLP Stable Expression Cell Line (A549) Copy Product Info
Catalog#： YOE-L064
Size： 1*10^6
Culture method： 90% F12K+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L064 Transcript NM_033035.5
Cell name TSLP Stable Expression Cell Line (A549) Species Human
Gene Name TSLP Passage ratio 1:3~1:4
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=1μg/ml
Culture method 90% F12K+10% FBS Mycoplasma test negative
Catalog
YOE-L064
Cell name
TSLP Stable Expression Cell Line (A549)
Gene Name
TSLP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Transcript
NM_033035.5
Species
Human
Antibiotic Concentration
Puro=1μg/ml
Mycoplasma test
negative
Culture method
90% F12K+10% FBS

Product Description

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.
Gene Expression Validation
No. Sample hTSLP-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 TSLP Stable Expression Cell Line (A549) 15.938 14.858 1.080 -18.876 481107.579
2 A549_Ctrl(Puro) 34.17 14.214 19.956 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 1818
FGA 2323
D2S1338 2424
D21S11 2929
D18S51 1417
D8S1179 1314
D3S1358 1616
D6S1043 1113
PENTAE 711
D19S433 1313
PENTAD 99
D1S1656 1718.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
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    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Citations:

[1] Yao, Jie, et al. "Long noncoding RNAs AC026904. 1 is essential for TGF‐β‐induced migration and epithelial‐mesenchymal transition through functioning as an enhancer of Slug in lung cancer cells." Environmental toxicology 35.9 (2020): 942-951.

[2] Roussel, L., Pham-Huy, A., Yu, A.C. et al. A Novel Homozygous Mutation Causing Complete TYK2 Deficiency, with Severe Respiratory Viral Infections, EBV-Driven Lymphoma, and Jamestown Canyon Viral Encephalitis. J Clin Immunol 43, 2011–2021 (2023).

[3] Khaodee,et al. "Transcriptomic analysis of glucosidase II beta subunit (GluIIß) knockout A549 cells reveals its roles in regulation of cell adhesion molecules (CAMs) and anti-tumor immunity." BMC Genomics 25, 82 (2024).

[4] Ding, Rui, et al. "TLR2 regulates Moraxella catarrhalis adhesion to and invasion into alveolar epithelial cells and mediates inflammatory responses." Virulence 15.1 (2024): 2298548.

[5] Yaman, Serkan, et al. "Targeted chemotherapy via HER2-based chimeric antigen receptor (CAR) engineered T-cell membrane coated polymeric nanoparticles." Bioactive Materials 34 (2024): 422-435.

[6] Wang, Liuxiang, et al. "DEPDC1 is a potential therapeutic target in lung adenocarcinoma." Nano Today 56 (2024): 102249.

[7] Wang, Juhong, et al. "Acetate reprogrammes tumour metabolism and promotes PD-L1 expression and immune evasion by upregulating c-Myc." Nature Metabolism (2024): 1-18.

Product Catalog#：YOE-L064
Product Name：TSLP Stable Expression Cell Line (A549)
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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